Welcome to the submission system for the access to the
Italian Structural Biology services
Access form for the CERM/CIRMMP Protein Isotope Labelling
Fields marked with
*
are required
Project
*
Title of Project
Please enter the project title.
*
Principal Applicant
*
First Name
Please enter the principal applicant first name.
*
Last Name
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*
Nationality
Please enter the principal applicant nationality.
*
Institution
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Phone Number
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Email
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Remember to also fill out the Visiting Scientist section below
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Visiting Scientist
Flag if the Applicant and Visiting scientist are the same person.
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First Name
Please enter the visiting scientist first name.
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Last Name
Please enter the visiting scientist last name.
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Nationality
Please enter the visiting scientist nationality.
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Institution
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Phone Number
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Email
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Project Proposal
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Project goals, background information and time planning (max 2500 chars)
Please enter the project goals....
*
Project status (results, related pubblications, grant applications) (max 2500 chars)
Please enter the project status.
*
Expected results (max 2500 chars)
Please enter the expected results.
Additional information (Document format accepted: pdf, doc, docx, odt - max size 5MB)
Protein Information
*
Protein Name
Please enter the protein name.
*
Gene Name
Please enter the genie name.
*
Species/Organism
Please enter the species/organism.
a.a. Sequence
*
Select Expression E.coli Strain and vector
Bl21 (DE3)
BL21-Gold(DE3)
BL21-Gold(DE3)pLysS
C41(DE3)
C41(DE3) + pEC 86
BL21 CodonPlus(DE3)-RIPL
Origami B(DE3)pLysS
Origami 2(DE3)pLysS
Rosetta 2(DE3)pLysS
Other strains available in lab
Tuner (DE3)
C41(DE3) pLysS
Other competent cell strains are available on demand
*
Expression Vector
Please enter the expression system vector.
Other information
*
Protein yield in rich medium (mg/L)
Please enter the protein yeld.
Required purity:
90%
80-70%
50-60%
Culture medium used for protein expression
Purification with tag (specify tag type and cleavage site, if present):
N-term fusion tag
C-term fusion tag
No Tag
*
Labelling:
15
N
13
C
15
N-
13
C
Expression and purification protocol (Document format accepted: pdf, doc, docx, odt - max size 5MB)
*
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Instruct-IT
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, and I am of legal age.
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